4TLX

Kutzneria sp. 744 ornithine N-hydroxylase, KtzI-FADred-NADP+-L-orn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-21
Detector	PSI PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	84.150, 156.906, 163.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.819 - 2.230
R-factor	0.1879
Rwork	0.186
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s5w
RMSD bond length	0.010
RMSD bond angle	1.205
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.000	2.270
High resolution limit [Å]	2.230	2.230
Number of reflections	106307
<I/σ(I)>	20.5	2.7
Completeness [%]	99.0	98.8
Redundancy	4.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.9-1.15 M potassium thiocyanate, 22-25% PEG 3350, 0.1 M Bis-tris propane pH 8.5