4TJV
Crystal structure of protease-associated domain of Arabidopsis vacuolar sorting receptor 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2011-08-28 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.600, 62.680, 35.810 |
Unit cell angles | 90.00, 109.39, 90.00 |
Refinement procedure
Resolution | 33.780 - 1.651 |
R-factor | 0.1675 |
Rwork | 0.166 |
R-free | 0.19020 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.352 |
Data scaling software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.780 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.026 | 0.148 |
Number of reflections | 15984 | |
<I/σ(I)> | 74 | 19.1 |
Completeness [%] | 97.7 | 94.9 |
Redundancy | 15.5 | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 10% glycerol, 0.1 M MES pH 6.0, 30% PEG 600, 5% (w/v) PEG 1000. |