4S2B
Covalent complex of E. coli transaldolase TalB with tagatose-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.308, 91.699, 130.987 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.120 - 1.460 |
| R-factor | 0.1536 |
| Rwork | 0.152 |
| R-free | 0.18460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1onr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.359 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.120 | 1.480 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.079 | 0.950 |
| Number of reflections | 140870 | |
| <I/σ(I)> | 10.6 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.8 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.2 | 0.15 M lithium sulfate, 18% PEG3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.2K |
