4RLV
Crystal Structure of AnkB 24 Ankyrin Repeats in Complex with AnkR Autoinhibition Segment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | H 3 2 |
Unit cell lengths | 179.685, 179.685, 304.856 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.590 - 3.494 |
R-factor | 0.2221 |
Rwork | 0.220 |
R-free | 0.25270 |
Structure solution method | SIRAS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.545 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
High resolution limit [Å] | 3.494 | 9.480 | 3.494 |
Rmerge | 0.121 | 0.055 | 0.783 |
Number of reflections | 23268 | ||
<I/σ(I)> | 9.3 | ||
Completeness [%] | 96.0 | 91.3 | 97.2 |
Redundancy | 10.2 | 9.7 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 0.5 M ammonium sulfate, 1.0 M lithium sulfate, and 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |