4RHM

Crystal structure of T. brucei arginase-like protein quadruple mutant S149D/R151H/S153D/S226D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	C 1 2 1
Unit cell lengths	82.123, 137.350, 87.778
Unit cell angles	90.00, 102.28, 90.00

Refinement procedure

Resolution	42.880 - 1.950
R-factor	0.203
Rwork	0.201
R-free	0.23700
Structure solution method	PHASER
Starting model (for MR)	4rhk
RMSD bond length	0.004
RMSD bond angle	0.778
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.950	4.340	1.950
Rmerge	0.091
Number of reflections	64646
<I/σ(I)>	17.1
Completeness [%]	94.3	99.8	73.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	0.2 M KNO3, 20% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K