4REZ
Crystal structure of the Middle-East respiratory syndrome coronavirus papain-like protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.28294 |
| Spacegroup name | P 63 |
| Unit cell lengths | 137.938, 137.938, 57.704 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.150 - 2.800 |
| R-factor | 0.2357 |
| Rwork | 0.231 |
| R-free | 0.27420 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.604 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.150 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 19513 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 18% PEG 4000, 0.1 M trisodium citrate, 16% isopropanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
