4RDW

The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-Guanidino-L-Glutaric acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	I 41 2 2
Unit cell lengths	133.223, 133.223, 124.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.599 - 1.591
R-factor	0.1498
Rwork	0.149
R-free	0.16480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.090
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.599
High resolution limit [Å]	1.591
Number of reflections	74771
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.1M SODIUM MALONATE, 0.1M HEPES, 0.5% JEFFAMINE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K