4RD1
Structure of aIF2-gamma H97A variant from Sulfolobus solfataricus bound to GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.320, 60.610, 144.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.112 - 1.500 |
R-factor | 0.161 |
Rwork | 0.160 |
R-free | 0.18900 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4rcz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.124 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.8.4_1496) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.400 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.136 | 0.533 |
Number of reflections | 65993 | |
<I/σ(I)> | 8.66 | 2.25 |
Completeness [%] | 99.7 | 98.4 |
Redundancy | 6.42 | 7.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 70% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |