4RA6
Crystal structure of linker less Pyrococcus furiosus L-asparaginase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-05-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 61.427, 61.427, 155.934 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.945 - 2.503 |
R-factor | 0.1971 |
Rwork | 0.193 |
R-free | 0.26350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wls |
RMSD bond length | 0.008 |
RMSD bond angle | 1.161 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.126 | 0.762 |
Number of reflections | 19946 | |
<I/σ(I)> | 13.3 | 1.53 |
Completeness [%] | 95.6 | 76.6 |
Redundancy | 4.6 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 15%(v/v) Reagent akcohol, 100mM Imidazole/HCl, 200mM MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |