4R5J
Crystal structure of the DnaK C-terminus (Dnak-SBD-A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0750 |
Spacegroup name | P 1 |
Unit cell lengths | 44.658, 69.412, 75.771 |
Unit cell angles | 102.12, 104.20, 92.96 |
Refinement procedure
Resolution | 44.248 - 2.361 |
R-factor | 0.2176 |
Rwork | 0.215 |
R-free | 0.25820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.065 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.076 | 0.497 |
Number of reflections | 34436 | |
<I/σ(I)> | 15.9 | 3 |
Completeness [%] | 98.0 | 97.4 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15 M potassium bromide, 30% w/v polyethylene glycol monomethyl ether 2000, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |