4QR1
Crystal structure of Streptococcus pyogenes Cas2 at pH 6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 55.054, 55.054, 117.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.830 - 2.193 |
R-factor | 0.21026 |
Rwork | 0.207 |
R-free | 0.26656 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4es2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.677 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.193 | 2.193 |
Rmerge | 0.538 | |
Number of reflections | 9551 | |
<I/σ(I)> | 15.55 | 6.58 |
Completeness [%] | 96.8 | 100 |
Redundancy | 12.8 | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% (w/v) PEG 8000, 200mM Na acetate, 100mM Na cacodylate pH 6.5 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |