4QR0
Crystal structure of Streptococcus pyogenes Cas2 at pH 5.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-20 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 54.803, 54.803, 124.013 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 2.400 |
R-factor | 0.19863 |
Rwork | 0.196 |
R-free | 0.24623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4es2 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.637 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.624 | |
Number of reflections | 7806 | |
<I/σ(I)> | 20.51 | 4.43 |
Completeness [%] | 98.1 | 97.8 |
Redundancy | 13.6 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30% (w/v) PEG 4000, 200mM ammonium acetate, 100mM tri-sodium citrate pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |