4QIE
Crystal Structure of PduA with edge mutation K26D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | .9795 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 108.110, 108.110, 334.340 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.847 - 2.350 |
R-factor | 0.2052 |
Rwork | 0.201 |
R-free | 0.23840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ngk |
RMSD bond length | 0.009 |
RMSD bond angle | 1.282 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX ((phaser-mr)) |
Refinement software | PHENIX ((phenix.refine: dev_1555)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.850 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.116 | 0.553 |
Number of reflections | 46445 | |
<I/σ(I)> | 12.09 | 2.64 |
Completeness [%] | 94.4 | 84.9 |
Redundancy | 6.87 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 0.1M Tri-sodium citrate, 2.4M ammonium sulfate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |