4Q5P
Lysine-Ligated Yeast Iso-1 Cytochrome C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0781 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 106.020, 106.020, 149.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.520 - 1.870 |
R-factor | 0.17386 |
Rwork | 0.172 |
R-free | 0.20367 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yea |
RMSD bond length | 0.009 |
RMSD bond angle | 1.228 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.520 | 3.190 | 1.970 |
High resolution limit [Å] | 1.870 | 2.780 | 1.870 |
Rmerge | 0.072 | 0.060 | 0.597 |
Number of reflections | 35544 | ||
<I/σ(I)> | 29.96 | 42.88 | 4.3 |
Completeness [%] | 99.9 | 100 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M ammonium phosphate, pH 8.1, 30% w/v PEG300, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |