4PLB
Crystal Structure of S.A. gyrase-AM8191 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.310, 93.310, 410.197 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.120 - 2.690 |
| R-factor | 0.1788 |
| Rwork | 0.176 |
| R-free | 0.22000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Refinement software | BUSTER (2.9.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 410.200 | 2.840 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.083 | 0.542 |
| Number of reflections | 55628 | |
| <I/σ(I)> | 23.3 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15.33 % PEG MME 5K, 50 mM Bis-Tris pH 6.0 |
