4PKE

The structure of a conserved Piezo channel domain reveals a novel beta sandwich fold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0716
Spacegroup name	H 3 2
Unit cell lengths	72.588, 72.588, 241.551
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.090 - 2.500
R-factor	0.229
Rwork	0.228
R-free	0.23800
RMSD bond length	0.006
RMSD bond angle	1.229
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.090	2.570
High resolution limit [Å]	2.450	2.450
Rmerge	0.093	0.810
Number of reflections	9377
<I/σ(I)>	17.7	3
Completeness [%]	99.9	100
Redundancy	10.1	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M phosphate-citrate buffer pH 4.0, 26% PEG1000, and 0.2 M Li2SO4