4PKD
U1-70k in complex with U1 snRNA stem-loops 1 and U1-A RRM in complex with stem-loop 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-22 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 80.220, 66.600, 93.730 |
Unit cell angles | 90.00, 110.95, 90.00 |
Refinement procedure
Resolution | 87.000 - 2.500 |
R-factor | 0.19992 |
Rwork | 0.197 |
R-free | 0.25791 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1urn |
RMSD bond length | 0.019 |
RMSD bond angle | 1.910 |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 87.540 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.076 | 0.350 |
Number of reflections | 15967 | |
<I/σ(I)> | 11 | 3.2 |
Completeness [%] | 99.1 | 99.4 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 40% MPD, 0.15 M sodium chloride, 0.1 M sodium acetate, pH 4.2 |