4PJW

crystal structure of human Stromal Antigen 2 (SA2) in complex with Sister Chromatid Cohesion protein 1 (Scc1), with bound MES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	78.733, 108.045, 180.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.705 - 2.850
R-factor	0.2104
Rwork	0.210
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pju
RMSD bond length	0.005
RMSD bond angle	0.869
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.098	1.000
Number of reflections	37638
<I/σ(I)>	20.1	1.5
Completeness [%]	99.9	99.9
Redundancy	8.8	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.12 M Morpheus Divalents Mix, 0.1 M Morpheus Buffer System 1, and 27-30% (v/v) Morpheus EOD_P8K (Molecular Dimensions).