4PCB
Conjugative Relaxase TrwC in complex with mutant OriT Dna
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2008-11-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 65 |
Unit cell lengths | 148.690, 148.690, 77.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 66.596 - 2.500 |
R-factor | 0.2572 |
Rwork | 0.255 |
R-free | 0.29440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omh |
RMSD bond length | 0.011 |
RMSD bond angle | 1.447 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.600 | 2.570 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 36116 | |
<I/σ(I)> | 15.5 | 5.5 |
Completeness [%] | 98.8 | |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 200 mM NaCl, 20 mM TrisHCl [pH 7.5], 1 mM dithiothreitol [DTT], 0.5 mM ethylenediaminetetraacetic acid [EDTA] |