4PBZ

Structure of the human RbAp48-MTA1(670-695) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-08-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	61.800, 59.820, 68.070
Unit cell angles	90.00, 99.19, 90.00

Refinement procedure

Resolution	20.000 - 2.150
R-factor	0.189
Rwork	0.186
R-free	0.23080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xu7
RMSD bond length	0.013
RMSD bond angle	1.494
Data reduction software	MOSFLM
Data scaling software	Aimless (0.1.16)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.600	33.600	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.057	0.023	0.538
Rpim	0.035	0.015	0.388
Total number of observations	94637	949	4598
Number of reflections	26112
<I/σ(I)>	13	34.3	2.1
Completeness [%]	97.3	90.9	80.8
Redundancy	3.6	3.2	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v 2-Methyl-2,4-pentanediol (MPD), 0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M (RS)-1,2-propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol, and 0.1 M MES/imidazole pH 6.5.