4PAY
Crystal structure of an N-terminal fragment of the Legionella pneumophila effector protein SidC.
Replaces: 4OM6Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-31 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.460, 107.584, 177.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 92.009 - 2.770 |
R-factor | 0.2022 |
Rwork | 0.199 |
R-free | 0.24640 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.989 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 92.009 |
High resolution limit [Å] | 2.770 |
Number of reflections | 52258 |
<I/σ(I)> | 17.8 |
Completeness [%] | 99.8 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277.15 | 8% PEG 8000, 0.2M barium chloride, 0.1M Hepes, pH 7.4 |