4P9R
Speciation of a group I intron into a lariat capping ribozyme (Heavy atom derivative)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.10530; 1.10564; 1.09553; 1.11525 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.440, 86.260, 109.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.945 - 2.703 |
R-factor | 0.2121 |
Rwork | 0.210 |
R-free | 0.25640 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.504 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.900 |
High resolution limit [Å] | 2.700 |
Number of reflections | 29825 |
<I/σ(I)> | 12.38 |
Completeness [%] | 99.9 |
Redundancy | 6.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | (i) 0.2 Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25% w/v PEG 3,350 or (ii) 0.2 Sodium chloride, 0.1 M HEPES pH 7.5, 25% w/v PEG 3,350 |