4P9K
CRYSTAL STRUCTURE OF A TRAP PERIPLASMIC SOLUTE BINDING PROTEIN FROM VERMINEPHROBACTER EISENIAE EF01-2 (Veis_3954, TARGET EFI-510324) A NEPHRIDIAL SYMBIONT OF THE EARTHWORM EISENIA FOETIDA, BOUND TO D-ERYTHRONATE WITH RESIDUAL DENSITY SUGGESTIVE OF SUPERPOSITION WITH COPURIFIED ALTERNATIVE LIGAND.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-21 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 76.290, 76.290, 110.813 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.535 - 1.400 |
R-factor | 0.1489 |
Rwork | 0.148 |
R-free | 0.16800 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.202 |
Data reduction software | MOSFLM (0.1.27) |
Data scaling software | Aimless |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 110.810 | 110.810 | 1.420 |
High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
Rmerge | 0.127 | 0.070 | 2.287 |
Rpim | 0.032 | 0.019 | 0.579 |
Total number of observations | 1228244 | 7163 | 59168 |
Number of reflections | 142034 | ||
<I/σ(I)> | 16 | 46.1 | 1.7 |
Completeness [%] | 100.0 | 99.2 | 100 |
Redundancy | 16.6 | 13.5 | 16.4 |
CC(1/2) | 0.999 | 0.998 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Protein (42.92 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT, 1 mM D-Erythronate); Reservoir ( 0.1 M Tris pH 8.5 2.4 M di-Ammonium Phosphate ); Cryoprotection (Reservoir + 20% glycerol |