4P87
Crystal structure of Est-Y29, a novel penicillin-binding protein/beta-lactamase homolog from a metagenomic library
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.731, 121.731, 155.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.999 |
| R-factor | 0.17944 |
| Rwork | 0.178 |
| R-free | 0.21224 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.999 |
| Number of reflections | 76224 |
| <I/σ(I)> | 28.1 |
| Completeness [%] | 99.8 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 287 | 1 M sodium citrate and 100 mM sodium acetate, pH 4.6 |
