4P2J
Crystal structure of the mouse SNX19 PX domain with bound sulphate ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.172, 32.987, 82.509 |
Unit cell angles | 90.00, 107.25, 90.00 |
Refinement procedure
Resolution | 61.287 - 2.400 |
R-factor | 0.2208 |
Rwork | 0.219 |
R-free | 0.26340 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.012 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.800 | |
High resolution limit [Å] | 2.400 | |
Rmerge | 0.097 | |
Number of reflections | 13235 | |
<I/σ(I)> | 13.4 | 3.4 |
Completeness [%] | 99.4 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG3350, 0.25 M NaCl, 5% glycerol, 0.1 M Tris (pH 8.0) |