4OZF
JR5.1 protein complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.930, 84.840, 109.680 |
Unit cell angles | 90.00, 92.99, 90.00 |
Refinement procedure
Resolution | 60.470 - 2.700 |
R-factor | 0.1864 |
Rwork | 0.184 |
R-free | 0.23840 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.140 |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 60.470 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.106 |
Number of reflections | 32383 |
<I/σ(I)> | 5.87 |
Completeness [%] | 97.8 |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | Mother liquor: 0.15 - 0.25M CaOAc, 0.1M Tris/HCl, 12-18% PEG3350, Additives:2-3 mM reduced and oxidised Glutathione |