4OYU

Crystal structure of the N-terminal domains of muskelin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	63.060, 65.300, 101.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.800 - 1.800
R-factor	0.158
Rwork	0.156
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.422
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.800	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	39509
<I/σ(I)>	17	4.7
Completeness [%]	100.0	100
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM BisTris pH 5.5, 200 mM sodium chloride and 32.5% (w/v) PEG 3350