4OYS
CRYSTAL STRUCTURE OF VPS34 IN COMPLEX WITH SAR405.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-07 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.810, 145.700, 61.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.850 - 2.900 |
Rwork | 0.180 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in-house |
Data reduction software | PROCESS |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.910 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 18671 | |
<I/σ(I)> | 8.8 | 1.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.5 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.9 M ammonium sulfate, 100 mM Tris pH 8.5 |