4OWR

Vesiculoviral matrix (M) protein occupies nucleic acid binding site at nucleoporin pair Rae1-Nup98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97950
Spacegroup name	P 4 21 2
Unit cell lengths	141.372, 141.372, 78.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.320 - 3.150
R-factor	0.21671
Rwork	0.213
R-free	0.28889
RMSD bond length	0.008
RMSD bond angle	1.067
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.150
Number of reflections	14247
<I/σ(I)>	15.5
Completeness [%]	97.2
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1 M HEPES pH 7.5, 11% PEG 10, 000, and 10% MPD