4OMY
Crystal Structure of SeMet NolR from Sinorhizobium fredii in complex with oligo AT DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 |
Unit cell lengths | 131.019, 131.019, 67.688 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.886 - 3.062 |
R-factor | 0.1339 |
Rwork | 0.132 |
R-free | 0.17810 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.452 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.900 |
High resolution limit [Å] | 3.050 |
Number of reflections | 22454 |
<I/σ(I)> | 24.8 |
Completeness [%] | 91.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 20% PEG-3350, 0.1 M sodium citrate/citric acid, and 0.2 M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |