4O18
Structural Basis for Resistance to Diverse Classes of NAMPT Inhibitors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2013-01-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.973973 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.308, 105.782, 82.821 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 82.350 - 1.920 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.350 | 1.927 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 77889 | |
| Completeness [%] | 98.9 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 292 | 0.1M Sodium phosphate, pH 8.6, 25% polyethylene glycol 3350, 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
