4NSK
CRYSTAL STRUCTURE OF THE MURINE CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX H-2DB IN COMPLEX WITH LCMV-DERIVED GP33 ALTERED PEPTIDE ligand V3P
Replaces: 3TBXExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.826 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.460, 108.540, 57.390 |
| Unit cell angles | 90.00, 122.43, 90.00 |
Refinement procedure
| Resolution | 19.090 - 2.600 |
| R-factor | 0.21925 |
| Rwork | 0.216 |
| R-free | 0.28297 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.319 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.900 | 2.710 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.552 | |
| Number of reflections | 14534 | |
| <I/σ(I)> | 6.9 | 1.7 |
| Completeness [%] | 94.0 | 88.2 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.6-1.8 M AMMONIUM SULFATE, 0.1 M TRIS HCL PH 7.0-9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
