4NIQ

Crystal Structure of Vps4 MIT-Vfa1 MIM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	190
Detector technology	CCD
Collection date	2013-04-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9998
Spacegroup name	P 21 21 21
Unit cell lengths	38.476, 56.711, 84.233
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.998 - 2.301
R-factor	0.2379
Rwork	0.236
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.399
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.044	0.023	0.336
Number of reflections	8662
<I/σ(I)>	14.7
Completeness [%]	99.9	98.8	100
Redundancy	7.1	6.4	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	26% PEG 4000, 10% (v/v) isopropanol, 0.1M HEPES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K