4NAS
The crystal structure of a rubisco-like protein (MtnW) from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-13 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97941 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.763, 175.671, 83.653 |
Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
Resolution | 29.500 - 1.920 |
R-factor | 0.1975 |
Rwork | 0.195 |
R-free | 0.25080 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.071 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.085 | 0.625 |
Number of reflections | 113403 | |
<I/σ(I)> | 22.8 | 2.3 |
Completeness [%] | 96.3 | 94.2 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M CalCl2, 20%(w/v) PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |