4MVZ
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.75 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.525, 177.825, 131.355 |
Unit cell angles | 90.00, 132.57, 90.00 |
Refinement procedure
Resolution | 29.900 - 3.300 |
R-factor | 0.24395 |
Rwork | 0.242 |
R-free | 0.28007 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.572 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.095 | 0.595 |
Number of reflections | 47359 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 95.9 | 97.3 |
Redundancy | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | CHAPSO:DMPC bicelles, 0.1M Na-Acetate, pH 4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |