4MVO
Structural Basis for Ca2+ Selectivity of a Voltage-gated Calcium Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Wavelength(s) | 1.75 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.475, 177.480, 130.960 |
Unit cell angles | 90.00, 132.63, 90.00 |
Refinement procedure
Resolution | 29.850 - 3.296 |
R-factor | 0.26088 |
Rwork | 0.260 |
R-free | 0.27923 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.547 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.480 |
High resolution limit [Å] | 3.296 | 3.296 |
Rmerge | 0.070 | 0.493 |
Number of reflections | 42145 | |
<I/σ(I)> | 2.5 | |
Completeness [%] | 93.9 | 84.4 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.75 | 298 | CHAPSO:DMPC BICELLES, 0.1M Na-Acetate, pH4.75, 2M Ammonium Sulfate, 28% Glucose, 15mM Calcium Chloride., VAPOR DIFFUSION, HANGING DROP, temperature 298K |