4MNT
Crystal structure of human DJ-1 in complex with Cu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-12-04 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.378 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.240, 75.240, 75.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.628 - 1.584 |
R-factor | 0.1521 |
Rwork | 0.151 |
R-free | 0.17740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.117 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.640 | 1.670 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.063 | 0.292 |
Number of reflections | 33930 | |
<I/σ(I)> | 7.5 | 2.6 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 15.5 | 14.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Sodium Malonate, 20% w/v PEG 3350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |