4MBZ
Structure of B-Lymphotropic Polyomavirus VP1 in complex with 3'-sialyllactosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2012-09-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.160, 97.240, 234.220 |
| Unit cell angles | 90.00, 96.21, 90.00 |
Refinement procedure
| Resolution | 48.750 - 1.750 |
| R-factor | 0.16625 |
| Rwork | 0.165 |
| R-free | 0.19485 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LPyV Native structure |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 333435 | |
| <I/σ(I)> | 14.6 | 2.3 |
| Completeness [%] | 99.1 | 98.1 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 12% (v/v) Isopropanol, 0.2 M calcium chloride, 0.1 M sodium acetate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
