4M9S
crystal structure of CED-4 bound CED-3 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97894 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 178.814, 178.814, 201.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.849 - 3.207 |
| R-factor | 0.2854 |
| Rwork | 0.283 |
| R-free | 0.33350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lqq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 56679 | |
| Completeness [%] | 74.6 | 27.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.6-0.8M sodium acetate, 0.1M HEPES (pH 7.5), 0.1M sodium fluoride , VAPOR DIFFUSION, HANGING DROP, temperature 291K |
