4LO0
Apo HA17-HA33
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97921 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 106.599, 118.680, 162.509 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.636 - 2.055 |
R-factor | 0.1893 |
Rwork | 0.188 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.214 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 2.200 |
High resolution limit [Å] | 2.055 | 2.055 |
Rmerge | 0.049 | 0.591 |
Number of reflections | 62896 | |
<I/σ(I)> | 13.7 | 2 |
Completeness [%] | 98.1 | 97.5 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.2 | 292 | 0.1 M MES, 0.1 M magnesium chloride, 5% PEG8000, pH 6.2, EVAPORATION, temperature 292.0K |