4LFB

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.765, 402.765, 177.085
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.537 - 3.009
R-factor	0.1711
Rwork	0.169
R-free	0.20340
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	0.785
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (dev_1119)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	3.120
High resolution limit [Å]	3.009	6.470	3.009
Rmerge	0.064	0.030	0.771
Number of reflections	274619
<I/σ(I)>	15.2
Completeness [%]	96.4	87.9	98.8
Redundancy	3.3	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	277	MPD, pH 6.5, hanging drop, temperature 277K