4LBA

Crystal structure of a conjugative transposon lipoprotein (BACEGG_03088) from Bacteroides eggerthii DSM 20697 at 1.70 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837, 0.97864, 0.97817
Spacegroup name	P 64
Unit cell lengths	126.923, 126.923, 39.352
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.217 - 1.700
R-factor	0.1662
Rwork	0.165
R-free	0.18730
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.573
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.217	25.217	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge		0.050	0.828
Total number of observations		3113	17712
Number of reflections	40202
<I/σ(I)>	12.6	11.4	0.9
Completeness [%]	99.8	97.4	100
Redundancy	6.2	6.4	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16.0% polyethylene glycol 3350, 0.2M magnesium chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K