4L3R

Crystal structure of a DUF4847 family protein (BACEGG_01241) from Bacteroides eggerthii DSM 20697 at 2.23 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97949,0.97886
Spacegroup name	P 21 21 21
Unit cell lengths	57.892, 59.174, 102.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.383 - 2.230
R-factor	0.1999
Rwork	0.198
R-free	0.23230
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XSCALE (July 4, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.383	29.383	2.310
High resolution limit [Å]	2.230	4.780	2.230
Rmerge	0.028	0.014	0.532
Number of reflections	17344	3198	3103
<I/σ(I)>	15.48	42.9	1.4
Completeness [%]	95.0	94.6	93.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M citric acid pH 5, 20% polyethylene glycol 6000, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K