4KWY
Crystal structure of a putative lipoprotein (CC_3750) from Caulobacter crescentus CB15 at 2.40 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97805, 0.91837, 0.97871 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 88.442, 136.351, 60.211 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.754 - 2.400 |
R-factor | 0.1942 |
Rwork | 0.192 |
R-free | 0.24120 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XSCALE (July 4, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.754 | 2.460 | |
High resolution limit [Å] | 2.400 | 10.730 | 2.400 |
Rmerge | 0.095 | 0.027 | 0.908 |
Number of reflections | 14362 | 198 | 1056 |
<I/σ(I)> | 12.2 | 37.2 | 1.8 |
Completeness [%] | 98.1 | 98.5 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.17M potassium dihydrogen phosphate, 20% polyethylene glycol 3350, 0.01M trimethylamine HCl, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |