4KP4
Deciphering cis-trans directionality and visualizing autophosphorylation in histidine kinases.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-27 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 163.686, 163.686, 130.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.580 - 3.000 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.286 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.580 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.074 | 0.939 |
Number of reflections | 20516 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 98.5 | |
Redundancy | 10.6 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 294 | 2% PEG 1000, 2% PEG 4000, 0.03M sodium acetate, Hepes pH 7.5, ammonium sulfate 1.5M, VAPOR DIFFUSION, SITTING DROP, temperature 294K | |
1 | 7.5 | 294 | 2% PEG 1000, 2% PEG 4000, 0.03M sodium acetate, Hepes pH 7.5, ammonium sulfate 1.5M, VAPOR DIFFUSION, SITTING DROP, temperature 294K |