4KM7
Human folate receptor alpha (FOLR1) at acidic pH, triclinic form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-18 |
Detector | SBC-3 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 33.709, 55.384, 69.504 |
Unit cell angles | 72.23, 86.95, 90.12 |
Refinement procedure
Resolution | 28.095 - 1.801 |
R-factor | 0.2242 |
Rwork | 0.222 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.029 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.100 | 0.073 | 0.180 |
Number of reflections | 43186 | ||
<I/σ(I)> | 12.2 | ||
Completeness [%] | 97.8 | 92.7 | 99.1 |
Redundancy | 3.4 | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1 M Citrate, pH 4.5, 0.1 M LiCl, 17.5 % PEG 8000, 40% (v/v) 2,2,2-trifluoroethanol, Vapor diffusion, hanging drop, temperature 298K |