4KLM
DNA polymerase beta matched product complex with Mg2+, 11 h
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.588, 79.760, 55.338 |
| Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
| Resolution | 23.833 - 1.747 |
| R-factor | 0.193 |
| Rwork | 0.188 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.099 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.747 | 1.747 |
| Rmerge | 0.053 | 0.277 |
| Number of reflections | 40346 | |
| <I/σ(I)> | 30.5 | 2.325 |
| Completeness [%] | 95.1 | 63.1 |
| Redundancy | 3.5 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |
