4KHX
Crystal structure of gp41 helix complexed with antibody 8062
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 106.814, 106.814, 98.537 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.952 - 2.921 |
| R-factor | 0.2393 |
| Rwork | 0.237 |
| R-free | 0.28950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.804 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 2.921 | 3.000 |
| Number of reflections | 13242 | |
| <I/σ(I)> | 20.47 | 1.6 |
| Completeness [%] | 94.4 | 63.7 |
| Redundancy | 6.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10% PEG4000, 20% isopropanol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
