4K3R
E. coli sliding clamp in complex with AcQLDLA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-08-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.960, 67.090, 81.180 |
Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
Resolution | 43.980 - 1.860 |
R-factor | 0.2116 |
Rwork | 0.209 |
R-free | 0.26390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mmi |
RMSD bond length | 0.006 |
RMSD bond angle | 1.068 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.265 | 43.985 | 1.960 |
High resolution limit [Å] | 1.859 | 5.880 | 1.860 |
Rmerge | 0.038 | 0.509 | |
Rmeas | 0.045 | 0.604 | |
Rpim | 0.024 | 0.323 | |
Total number of observations | 7367 | 21862 | |
Number of reflections | 61020 | ||
<I/σ(I)> | 9.7 | 20.7 | 2.4 |
Completeness [%] | 92.5 | 97.8 | 78.4 |
Redundancy | 3.1 | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 100mM MES, 100-150mM CaCl2, 25-30%(v/v) PEG400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |