4JPI
Crystal structure of a putative VRC01 germline precursor Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | H 3 |
Unit cell lengths | 172.440, 172.440, 92.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.149 - 2.100 |
R-factor | 0.1766 |
Rwork | 0.175 |
R-free | 0.21430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3NGB CHAINS H AND L |
RMSD bond length | 0.007 |
RMSD bond angle | 1.105 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.220 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 59809 | |
<I/σ(I)> | 13.6 | 2.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% w/v PEG3350, 0.2 M lithium citrate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |